We found upregulation of senescent markers SA-β-Gal, P53, P21, γ-H2AX, and COX2, and downregulation of Lamin B1 in cortical pyramidal neurons in CamKII:Pten–/– mice with status epilepticus, but not in CamKII:Pten–/– mice that had not developed seizures at 5 postnatal weeks or control Ptenfl/fl mice at 12 postnatal weeks (Figure 7, E–G). Here, H2AX is linked to status epilepticus.