TNFRSF4 and malignant colon neoplasm: To clarify the protumor role of OX40 in pan-cancer ECs, we analyzed open-access data from the Tumor Immune Single-cell Hub single-cell transcriptome database and identified several tumor types with high OX40 expression in the EC subgroup, especially in colon cancer, ovarian cancer, and glioma (Supplemental Figure 4A).