The cytotoxicity of BBζ and BBζ-Neo were measured by co-culturing them with MSLN-positive tumor cells (AsPC-1, MSLNOE PANC-1 or MSLNOE BxPC-3, Supplementary Fig. 3c) for 4 hours at an effector to target ratio (E:T) of 1:1 or 5:1. The gene discussed is MSLN; the disease is neoplasm.