To investigate the potential role of STK25 in the incidence of HCC, we analyzed STK25 mRNA abundance in human HCC tumors and nontumor liver tissues by querying the microarray datasets of 3 large cohorts from the Gene Expression Omnibus (GEO) database (GSE25097: N = 268 for tumor and N = 243 for nontumor; GSE14520: N = 225 for tumor and N = 220 for nontumor; GSE36376: N = 240 for tumor and N = 193 for nontumor) and the whole RNA sequencing (RNA-seq) data combing the Cancer Genome Atlas (TCGA) and the Genotype-Tissue Expression Portal (GTEx) (N = 371 for tumor and N = 160 for nontumor). This evidence concerns the gene STK25 and hepatocellular carcinoma.