Using classical Co-IP and immunoblotting techniques in the U2OS-rGR human osteosarcoma cell line, Dobrovolna et al. identified that the NCOA2 coregulator (the authors used the previous naming as GR-interacting protein 1), in addition to functioning as a general coactivator for GR, also selectively works to repress the activity of certain GR-mediated signaling pathways (126). This evidence concerns the gene NR3C1 and osteosarcoma.