Finally, we analysed the expression of these prioritised targets in CRC versus matched normal mucosa using the TNM-plot tool (https://tnmplot.com/analysis/) and we found that most of them show tumour-specific mRNA expression except for ACAT1 and CDC42, in contrast to the literature [31, 57] (Fig. S15). This evidence concerns the gene ACAT1 and colorectal carcinoma.