L1 RT burden was also higher in p53-mutant tumors (mean difference of 1.57 burden between log2-, QC-adjusted RT counts, p < 10−10, 2-sided Mann–Whitney U test), including comparisons within tumor types for lung squamous cell, head and neck, and colon cancers (Supplementary Fig. 9c, d). Here, TP53 is linked to neoplasm.