According to our analysis, the validation of the promoter methylation of hub genes is analyzed from TCGA Illumina bead chip data using UALCAN, which shows an inverse relation with gene expression profile, stating the significant statistical significance in COAD patients (AKT1, EGFR, IFNG, PXDN, H6PD, MYC, GSK3B, APP, NOTCH1, RPS27A) and READ patients (IFNG, PXDN, NOTCH1, GSK3B, TP53, MYC, PTEN, TGFB1, H6PD, IL-1β). The gene discussed is RPS27A; the disease is colon adenocarcinoma.