RFWD3 and osteosarcoma: Previous studies have established that NAD+ serves as a crucial substrate for poly (ADP‐ribose) polymerases (PARPs) during the DNA repair process, highlighting one of the mechanisms through which NAD+ contributes to cisplatin (DDP) resistance.[19] However, our integration of metabolomics data has revealed a pronounced downregulation of purine, aspartate, and glutamate metabolic pathways in RFWD3 knockout osteosarcoma cells compared to the control group (Figure6A).