The PP1 inhibitors CD2BP2 and WBP11 [46, 47], acting as CLK1 kinase enhancers, were upregulated in S‐III fractional ESCC and functioned in spliceosome assembly and pre‐mRNA splicing, and knockdown of CD2BP2 and WBP11 significantly inhibited the proliferation of ESCC cells. Here, WBP11 is linked to esophageal squamous cell carcinoma.