Furthermore, RT-qPCR analysis of HNSCC cell lines revealed a significant upregulation of EIF3D, EIF1, LARP1, and METTL1 mRNA expression in HNSCC cell lines (NH6, HSC3, and SCC9) compared to normal epithelial cells (Figure 14F). Here, EIF3D is linked to head and neck squamous cell carcinoma.