Furthermore, RT-qPCR analysis of HNSCC cell lines revealed a significant upregulation of EIF3D, EIF1, LARP1, and METTL1 mRNA expression in HNSCC cell lines (NH6, HSC3, and SCC9) compared to normal epithelial cells (Figure 14F). This evidence concerns the gene EIF1 and head and neck squamous cell carcinoma.