In immune cell analyses, T cells regulatory (Tregs), NK cells activated and Macrophages M0 were statistically significant.CCL5, IL6, TLR4, HLADQA1, IL1B, ITGAL, and CD86 were strongly associated with these three cells and showed inhibitory effects, and lower expression levels in NSCLC. The gene discussed is TLR4; the disease is non-small cell lung carcinoma.