Chromogenic IHC and high-plex immunofluorescence of pretreatment (day −4) and posttreatment (day +13) formalin-fixed paraffin-embedded tumor specimens confirmed increases in the number of CD3+ T cells (Fig. 2a,b), including CD3+CD4+ T cells (greater than tenfold increase in the posttreatment specimen relative to pretreatment (from 0.3% to 5%)) and CD3+CD8+ cytotoxic T cells (eightfold increase posttreatment relative to pretreatment (from 0.4% to 3%); Fig. 2b). This evidence concerns the gene CD8A and neoplasm.