NFKB1 and mesothelioma: Next, we employed an ELISA EMSA test to assess the DNA-binding activity of each NF-κB subunits, in the different cell lines, revealing a notable activity of DNA binding of p65 and RelB, which was intensified upon TNF-α treatment in mesothelioma cell lines IST-Mes2 and MPP89 (Fig. 2B).