In mouse malignant melanoma cell lines B16F10 and Yummer1.7, knockdown of USP35 by shRNAs, which specifically targeted USP35 mRNA, significantly enhanced VSV virus-induced expression of inflammation and antiviral-associated factors, including IFNβ, CXCL10, and ISG15 (Supplementary Fig. 2A, B; Fig. 2C, D). This evidence concerns the gene ISG15 and melanoma.