PFKFB3 and colorectal carcinoma: In order to continue to explore the effect of PFKFB3, the target gene of miR-519d-3p on 5-FU sensitivity in CRC cells, the authors performed cellular experiments and found that: 1) The expression of PFKFB3 in CRC cells was higher than that in normal colorectal mucosa; 2) PFKFB3 could enhance the proliferation and reduce the apoptosis of CRC cells under the effect of 5-FU, and thus inhibit the sensitivity of CRC cells to 5-FU.