On this basis the authors added PFK-15, an inhibitor of PFKFB3, and the results showed that the negative regulation of PFKFB3 by miR519d-3p could be partially reversed with the use of an inhibitor of PFKFB3, which further demonstrated that miR519d-3p could target and regulate PFKFB3, and the authors then proposed the hypothesis that miR-519d-3p might promote CRC cells' sensitivity to 5-FU by targeting and binding to PFKFB3 and suppressing its expression to promote sensitization of CRC cells to 5-FU. This evidence concerns the gene PFKFB3 and colorectal carcinoma.