On the basis of the previous experiments, we proceeded to transfect CRC cells with miR-519d-3p mimics to inhibit its downstream target gene PFKFB3, while the transfected miR-519d-3p inhibitor produced the opposite effect, suggesting that miRNA-519d-3p binds PFKFB3 and regulates its expression; subsequently, the authors co-transfected PFKFB3 with miR-519d- 3p cotransfected into CRC cells, and the data showed that the upregulation of PFKFB3 partially reversed the effects of miR-519d-3p on proliferation, apoptosis, and invasion produced in CRC cells under the action of 5-FU. This evidence concerns the gene PFKFB3 and colorectal carcinoma.