Methods: This prospective study aimed to quantitatively measure PKM2 immunohistochemistry (IHC) expression in GBM (IDH wildtype) versus ASTROG4 (IDH R132H mutant), to assess the correlation between PKM2 expression and prognosis in these two patient groups, and to investigate the prognostic significance of ATRX and p53 expression in relation to PKM2 levels. The gene discussed is PKM; the disease is glioblastoma.