In this context, AEP deficiency in MDA-MB-231 cells resulted in increased protein phosphatase activity, as measured with 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP), which could be dampened by pretreating AEP KD samples with recombinant AEP (Fig. 4E), supporting a role of AEP in DNA damage repair response and cell cycle regulation through the regulation of protein phosphatase activity in BC cells. This evidence concerns the gene LGMN and breast cancer.