Subsequently, Suzuki et al. [50] discovered that a single CSD stimulation did not induce a significant rise in HMGB1 levels or microglial activation; however, repeated CSD stimulations resulted in substantial release of HMGB1 and subsequent microglial activation, suggesting the potential involvement of HMGB1 in the chronic progression rather than acute attacks of migraine. The gene discussed is HMGB1; the disease is migraine disorder.