Cluster #1 (12–16%) consisted primarily of non-seminomatous germ cell tumor (NSGCT), characterized by high expression of PRL, GNRH1, HSD17B2 and SRD5A1. Cluster #2 (42–50%) included predominantly seminomas with high expression of SRD5A3, being highly infiltrated by T and B cells. This evidence concerns the gene SRD5A3 and nongerminomatous germ cell tumor.