EGF and neoplasm: Using organoid medium containing advanced Dulbecco’s Modified Eagle Medium/Ham’s F-12 (DMEM/F12), B27 without vitamin A, N2, epidermal growth factor (EGF), FGF2, insulin-like growth factor 1 (IGF-1), and 0.5% BME, we generated eight tumoroid lines (“O” suffix to sample identifiers to indicate organoids; PS-01a-O, PS-01b-O, PS-01c-O, PS-01d-O, PS-02a-O, PS-02b-O, PS-02c-O, and PS-03b-O) of the nine tumor tissues that we placed in culture, achieving a success rate of 88.8% (8 of 9).