GJB2 and hearing loss disorder: As shown in the flowchart in Figure 1, NGS testing of the total cohort (n = 485, M:F = 250:235) revealed 35 children (M:F = 11:24) with GJB2 (n = 27, M:F = 9:18) and SLC26A4 (n = 8, M:F = 2:6) associated hearing loss.