Differential thermal profiling of total proteins from 22Rv1 PCa cells treated with 15 μm 433‐3β (24 h) demonstrated a significant shift in fraction of natively folded lanosterol synthase (LSS) and lanosterol 14‐alpha demethylase (CYP51A1) against a temperature gradient. The gene discussed is LSS; the disease is posterior cortical atrophy.