The proteomic analysis further substantiated thelipidomic findings via REIMS and MALDI-MSI, revealing several keybiomarkers and pathways associated with survival outcomes in GBM patients.Notably, we identified differential expressions of proteins involvedin the metabolism of proteins, the neuronal system, and lipid metabolism.The data showed that MBP, PLP1, and CNP were notably upregulated inthe short-term survival group. The gene discussed is PLP1; the disease is glioblastoma.