Inoculation of chickens with rMd5-Md5/CVI-Meq #1 and #2 resulted in 100% mortality (Figure 5A) and MD (Table 2), while mortality (Figure 5B) and MD incidence (Table 2) in chickens inoculated with rMd5-CVI/Md5-Meq #1 and #2 were 33 and 43%, respectively. This evidence concerns the gene RMND5A and Menkes disease.