In this study, TRIM38 overexpression in U251 cells increased the expression of RIG-I/MDA5 signaling pathway-related proteins, including RIG-I, MDA5, TBK1, and IRF3, at 6 h post-ZIKV infection, and also increased the IFN-β level. The gene discussed is IFNB1; the disease is Zika virus infectious disease.