TP53 and cancer: Cytotoxicity (MTT assay) was evaluated against a panel of cancer cell lines [A549 (lung); MCF7 (breast); VA13 (SV40-transformed lung); Hek293T (embryonic kidney); LNCaP, PC3 (prostate); in addition to HCT116+/+, HCT116−/− (colon positive, and negative p53)] (Supplementary Figure S13).