To determine whether the cytotoxic activity of rTBL-1 is dependent on EGFR in the cellular system, we evaluated the effect of a range of concentrations between 0 and 100 μg of rTBL-1/mL in two colon cancer cell types: MC-38 cells, EGFR+ [29], and CT-26, EGFR− [30]. The gene discussed is EGFR; the disease is malignant colon neoplasm.