Knowing that ZEB1 regulates the transcription of ITIH2, the additional test we performed with ZEB1 aimed to investigate its presence for two reasons: the first was to find out whether there is a relationship between ZEB1 and ITIH2 in canine mammary tumors, and the second was to confirm whether ZEB1 regulates the transcription of ITIH2 [38]. The gene discussed is ITIH2; the disease is breast cancer.