TP53 and lung carcinoma: At a high multiplicity of infection (MOI), HAdV5 robustly expressed E1A protein in both A549, a lung cancer cell line that is highly permissive to HAdV5 infection, and hTERT-RPE1 (Figure 1b), an immortalized cell line derived from retinal pigment epithelia that is frequently used to model normal p53 functions [21].