For the rs2073498 (Ala133Ser) variant in the RASSF1 gene, we observed that the patient carriers of Ala/Ser and Ser/Ser genotypes showed an increased susceptibility for developing CRC (OR = 2.58; 95% CI = 1.85–3.60, p = 0.001 and OR = 8.84; 95% CI = 3.62–21.56, p = 0.001), respectively, and this association was also evident under the dominant model of inheritance, Ala/Ser + Ser/Ser vs. Ala/Ala (OR = 2.93; 95% CI = 2.12–4.06, p = 0.001). This evidence concerns the gene RASSF1 and colorectal carcinoma.