MLH1 and colorectal carcinoma: Another study proposing regional analysis was conducted where five regions were analyzed: region A (−755 to −574), region B (−597 to −393), region C (−420 to −188), region D (−286 to −53), and region E (−73 to +86), reporting that 13 out of 210 patients with CRC showed total methylation of the MLH1 CpG island, mainly observed in MSI-H samples (13/13), in contrast to partial methylation, which was found in only two MSI-H samples (13/2) (p < 0.0001) [14].