The frequencies of the leukemia-specific interferon gamma (γ)-producing T cells after MLCM compared to MLCctr are given in Figure 2B. For the detection of interferon-γ-producing T cells, an intracellular cytokine assay (ICA) was used, and for the detection of degranulating CD107a-positive cells, a degranulation assay was used after MLCM compared to MLCctr (Figure 2C). This evidence concerns the gene IFNG and leukemia.