Click chemistry was sometimes utilized alone (using Click-iT palmitic acid with azide) to assess the palmitoylation of β-catenin in PTC cells [109], after metabolic labeling with 17-ODYA to assay for the tyrosinase palmitoylation state in HM3KO cells [97], and after cell labeling with palmitic acid azide to assay for Fas palmitoylation in HEK293T cells [130], as well as MCAM and CD44 in WM239A melanoma cells [114], or following cell labeling with Alk-14 to assess the palmitoylation state of FLAG-tagged claudin-3 in HEK293T cells [131]. This evidence concerns the gene TYR and melanoma.