The goal of this study was to examine the utility of HEK293 cells as a model of non-cancer cells for testing the efficiency of the cellular transport and biocompatibility of biotinylated, cationic, or neutral nanoparticles associated with transport via the sodium multivitamin transporter (SMVT) or monocarboxylate transporter 1 (MCT-1). Here, SLC16A1 is linked to cancer.