HES1 and female reproductive organ cancer: HES1 and CTNNB1 expression was significantly lower in high-grade (grade 3) gynecologic cancer compared to low-grade (grade 1) cases (p < 0.05, FC= 1.48, 1.22, respectively), while DLL1 and FBXW7 also showed a tendency for reduced expression in high-grade tumors (p = 0.05, FC = 1.17 and 1.13, respectively) (Supplementary Figure S4).