Moreover, 2 Hz EA not only relieved fibromyalgia pain but also decreased TRPV1 levels in the spinal cord (Figure 3A, 100.13 ± 3.32%, red column, # p < 0.05, n = 6), as did PD-L1 injection (Figure 3A, 101.16 ± 2.95%, red column, # p < 0.05, n = 6), and disappeared in the Trpv1 knock out (Figure 3A, 13.66 ± 1.09%, red column, # p < 0.05, n = 6). This evidence concerns the gene TRPV1 and fibromyalgia.