COL8A2 and Fuchs endothelial corneal dystrophy: In a study on mice with an FECD model, they demonstrated that a single intraocular injection of Ad encoding both the Cas9 gene and guide RNA (Ad-Cas9-Col8a2gRNA) effectively suppressed the expression of mutant COL8A2 in corneal endothelial cells, prevented endothelial cell loss, and restored the pumping function of corneal endothelium in adult mice [114].