To determine the precise pathway of LKB1-dependent cell death, RA FLSs were transfected with LKB1 siRNA or control siRNA and used western blot to measure the levels of the autophagy marker LC3A/B and GPX4 and SLC7A11, which suppress the ferroptosis pathway and are used as markers of ferroptosis (Figure 2A). The gene discussed is GPX4; the disease is rheumatoid arthritis.