ACTA2 and hydrops fetalis: In the preventative approach, hepatocyte EVs were administered upon initiation of CDAA-HF diet feeding over 4-, 8-, or 12-week periods and resulted in attenuation of diet-induced collagen deposition, ACTA2 expression, pro-fibrotic gene expression, infiltration of lymphocytes, and macrophages, as well as improvements in serum AST or triglyceride levels.