Since, in the present study, we also observed that the protective effect of CF, CGA and GSH-MEE against lipotoxicity correlated with increased lipid droplet formation, we next evaluated palmitate toxicity in primary steatotic rat hepatocytes (fat) isolated from ZDF diabetic rats (Fa/Fa), a leptin-deficient rat that develops spontaneous steatosis after 12 weeks, and hepatocytes isolated from lean Zucker rats (Fa/+). This evidence concerns the gene LEP and steatosis.