Considering all the aforementioned evidence, and the putative relationship between a secreted form of SMase, known as acid SMase (aSMase), leading to an increase in SM levels, and migraine, our study aims to elucidate the potential implication of aSMase in migraine to confirm the hypothesis that aSMase serum levels are elevated in EM and CM patients compared to healthy subjects, as well as the levels of SM. Here, SMPD1 is linked to migraine disorder.