Figure 3D displays the measured mRNA levels of IL-6, IL-1β, TNF-α, and IL-10. The M group showed significantly higher levels of IL-1β, IL-6, and TNF-α mRNA than the NC group, which proved increased inflammation in the colon. SLH treatment strongly decreased IL-1β and TNF-α mRNA levels in the samples according to statistical analysis (p < 0.05). The HD, HLH, and HLL treatments successfully lowered TNF-α mRNA expression levels (p < 0.05), which suggests that they can defend against colitis. The gene discussed is TNF; the disease is colitis.