After the PAS840 intervention, we explored PAS840’s protective effect and potential mechanism against the excitotoxicity caused by glutamate from aspects such as cell survival rate, intracellular Ca2+ level, oxidative stress level, and the influence on NMDAR1 expression, hoping to provide a basis for PAS840’s research on combating neurological diseases at the animal level. The gene discussed is GRIN1; the disease is nervous system disorder.