Sharkovska et al., [12] showed that riociguat (3 and 10 mg/kg/day) for 18 days improved kidney function and attenuated renal interstitial fibrosis in hypertensive renin-transgenic rats treated with L-NAME, and they also showed that riociguat (15 mg/kg/day) for 15 weeks reduced blood pressure and improved kidney function and attenuated renal fibrosis in rats with 5/6 nephrectomy. This evidence concerns the gene REN and renal fibrosis.