HDGF and colorectal carcinoma: To determine whether HDGF affects the sensitivity of CRC cells to chemotherapeutic agents, we treated HCT116 WT, HCT116 HDGF-KO, HT29 WT, and HT29 HDGF-KO cells with the commonly used CRC chemotherapy drugs 5-FU and oxaliplatin, and measured cell apoptosis using flow cytometry (Figure 5A,B).