Notably, gintonin-binding sites showed minimal co-localization with GFAP and Iba-1 marker proteins (less than 10%; Figure 1D,E, Figure 1G,H), although the numbers of Iba1- and GFAP-positive cells were significantly increased in patients with AD compared to healthy cortices (Figure 1F,I). This evidence concerns the gene GFAP and Alzheimer disease.