By bioinformatics analysis and experimental data obtained in this study, we found that the high expression of PHF8 was related to poor prognosis of CRC patients, inhibited antigen presentation and interferon response process, and antagonized the therapeutic effect of PD-1 antibody, which was consistent with a previous study showing that PHF8 could inhibit the intrinsic immune response [29]. Here, PHF8 is linked to colorectal carcinoma.