Further examining the immune-related factors associated with tumors, we found that PHF8 was negatively correlated with tumor mutation burden (TMB), microsatellite instability (MSI), M1 macrophages, CD4 + T cells, CD8 + T cells and NK cells, but positively correlated with M2 macrophages and neutrophils (Fig. 2A). The gene discussed is CD4; the disease is neoplasm.