Next, according to the basal levels of PHF8 in different CRC cell lines (Figure S9), we ectopically expressed or knocked down PHF8 in LOVO, SW480, HCT116, RKO, and MC38 cells (Fig. 2B-C), and determined their effect on the expression of the above immune-related genes. Here, PHF8 is linked to colorectal carcinoma.