Considering whether EBV could have an effect on the expression of PD-L1 on VECs, we first treated HUVECs with supernatants from EBV-positive and EBV-negative tumor cell lines (HK1-EBV, HK1, CNE2-EBV, CNE2, AGS-EBV, AGS). This evidence concerns the gene CD274 and neoplasm.