For example, single-nucleus RNA sequencing and bulk RNA sequencing data have identified ferroptosis and iron metabolism-related genes (FIRGs), including ACSL4 and CHMP5 (which encodes the chromatin-modifying protein-charged multivesicular body protein 5), are involved in the pathogenesis of ALS primarily by affecting calcium signaling pathways, synaptic pathways, and a variety of immune pathways.298 CHMP5 has also been shown to accumulate in the plasma membrane during ferroptosis, reducing lipid peroxidation and participating in the regulation of ferroptosis. Here, ACSL4 is linked to amyotrophic lateral sclerosis.