Tandem mass tag-based quantitative proteomics analysis in a mouse model of myocardial infarction revealed significant downregulation of the GSH metabolic pathway and various ROS pathways, particularly GPX4.338 Moreover, pharmacological inhibition of GPX4 and siRNA-mediated Gpx4 knockdown in H9c2 cells led to the accumulation of lipid peroxides. Here, GPX4 is linked to myocardial infarction.