Mechanistically, they found that EP1 triggers an increase in intracellular calcium and activates protein kinase C (PKC)/Nrf2 signaling, thereby protecting cardiomyocytes from DOX-induced ferroptosis by upregulating Nrf2-driven genes that encode antioxidant proteins such as GPX4 and SLC7A11.449 These findings implicate that activating the PGE2/EP1 pathway may protect cardiomyocytes from DOX-induced ferroptosis via PKC/Nrf2 signaling and could serve as an attractive target for the prevention and/or treatment of DOX-induced cardiomyopathy. This evidence concerns the gene GPX4 and cardiomyopathy.