Mice with iron overload showed delayed muscle regeneration, reduced size of regenerated muscle fibers, decreased expression of myoblast differentiation markers, and decreased phosphorylation of mitogen-activated protein kinase (MAPK) signaling pathway after CTX-induced muscle injury.101 In addition, iron deficiency also reduces myoglobin expression and mitochondrial oxygen-consuming capacity, thereby reducing myoblast proliferation.79 Therefore, the imbalance of iron metabolism may destroy the ability of satellite cell proliferation, which may damage their ability to regenerate muscle tissue. This evidence concerns the gene MB and Iron deficiency anemia.